Both natural and synthetic approaches have

2018-10-20

Both natural and synthetic approaches have been used to generate fully defined hPSC culture substrates. ECM proteins such as vitronectin and laminins 511 and 521 have been shown to maintain hESC pluripotency and self-renewal (Braam et al., 2008; Domogatskaya et al., 2012; Miyazaki et al., 2012; Rodi

br Experimental Procedures br Author Contributions br Acknow

2018-10-20

Experimental Procedures Author Contributions Acknowledgments Introduction Nuclear reprogramming of somatic purchase EPZ 005687 using genetic modification and overexpression of pluripotent transcription factors is essential for creating induced pluripotent stem cells (iPSCs) in vitro (Tak

br Experimental Procedures Murine ESC lines E Tg A

2018-10-20

Experimental Procedures Murine ESC lines E14Tg2A (passages 25–38) and K/l EB5 (transgenic Rax-GFP ESCs, used with the kind permission of Y. Sasai, RIKEN Center) and H9 hESCs (WiCell) were cultured as described (Bertacchi et al., 2013; Lupo et al., 2013). All animal protocols were reviewed and app

br Introduction In mammals cells from the blastocyst stage c

2018-10-20

Introduction In mammals, cells from the GSK J4 stage can be used to form embryonic stem (ES) cell lines that can self-renew in culture while maintaining the ability to differentiate into cells of the three germ layers (Reubinoff et al., 2000). Directed in vitro differentiation protocols generall

glucose transport proteins br Results br Discussion The plur

2018-10-20

Results Discussion The pluripotency status of ES glucose transport proteins or iPS cells is maintained by a tightly regulated transcription factor network (Boyer et al., 2005). OCT4 is at the center of this network (Pardo et al., 2010; van den Berg et al., 2010) and is associated with chromati

Because Q SA and Q SC represent an

2018-10-20

Because Q3SA and Q3SC represent an isogenic pair of iPSC lines from the same individual but with contrasting ATM genotypes (ATM−/− versus ATM+/−, respectively), we examined gene expression differences using RNA sequencing (RNA-seq), evaluating matches at the transcript level. Relatively few mRNAs we

We obtained some evidence that NMD

2018-10-20

We obtained some evidence that NMD not only influences hESC fate through TGF-β and BMP signaling, but also through the WNT, FGF, and NOTCH pathways (Figure 2E). This raises the possibility that the ability of NMD to influence 5-lipoxygenase and mesoderm differentiation is a combinatorial process am

In this study we tested the hypothesis that high RAD

2018-10-20

In this study, we tested the hypothesis that high RAD51 (-)-JQ1 and RAD51 foci activation is specifically associated with GSCs and that small-molecule inhibitors are effective GSC radiosensitizers. Results Discussion Identifying targets in the DDR pathway as a means to sensitize cancers to DN

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2018-07-29